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What is the primary objective of this study?

Serum proteomics is a very useful tool to identify various disease. The purpose of the present study was to find differential proteins among patient with normal, SIRS, sepsis, severe sepsis, death and to screen potential biomarkers for their dynamic changes. Serum proteins were identified by iTRAQ labeling and LC-MS/MS. The bioinformatics analysis was performed with the Mascot software and the International Protein Index (IPI) and the Gene Ontology (GO) Database and KEGG pathway Database. The differentially expressed proteins were verified by Western blot by another sample collected from clinical.

Who is eligible to participate?

Inclusion Criteria: - Male and female aged 18 years old and over; - clinically confirmed infection; - fulfilled at least two criteria of systemic inflammatory response syndrome - (a) core temperature higher than 38 °C or lower than 36 °C - (b)respiratory rate above 20/min, or PCO2 below 32 mmHg - (c) pulse rate above 90/min, and - (d) white blood cell count greater than 12,000/μl or lower than < 4,000/μl or less than 10% of bands. Exclusion Criteria: - younger than 18 years of age; - acquired immunodeficiency syndrome; - reduced polymorphonuclear granulocyte counts (< 500 μL-1); - died within 24h after admission into the ICU, or refused to participate in the study, or declined treatment during the period of observation.

Which medical condition, disease, disorder, syndrome, illness, or injury is researched?

SIRS

Sepsis

Severe Sepsis

Death

Study Interventions

Interventions can include giving participants drugs, medical devices, procedures, vaccines, and other products that are either investigational or already available or noninvasive approaches such as surveys, education, and interviews.

Study Arms

Research studies and clinical trials typically have two or more research arms. An arm is a group of people who receive the same treatment in the study.

Normalnormal person under physical examination

SIRStemperature >38 ℃ or <36℃; pulse rate>90 beats/min; ventilatory rate>20 breaths/min or hyperventilation with partial pressure of arterial carbon dioxide (PaCO2)<32mmHg; white blood cell count>12,000μL-1 or <4000μL-1 or >10% immature cells

spesissepsis is defined as SIRS plus confirmed infection.

severe sepsissevere sepsis: sepsis associated with organ dysfunction, hypoperfusion, or hypotension; septic shock: sepsis with arterial hypotension, despite adequate fluid resuscitation.

deathsepsis patients within 48 hours before death

Study Status

Unknown status

Start Date: May 2010

Completed Date: March 2012

Phase:

Type: Observational

Design:

Primary Outcome: Survival status

Secondary Outcome:

Study sponsors, principal investigator, and references

Principal Investigator: Lixin Xie, MD

Lead Sponsor: Chinese PLA General Hospital

Collaborator:

More information:https://clinicaltrials.gov/show/NCT01493466